2B2T

Tandem chromodomains of human CHD1 complexed with Histone H3 Tail containing trimethyllysine 4 and phosphothreonine 3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-08
Detector	MARRESEARCH
Wavelength(s)	0.97926, 0.97939, 0.97240
Spacegroup name	C 1 2 1
Unit cell lengths	111.986, 54.686, 100.989
Unit cell angles	90.00, 112.15, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.218
Rwork	0.218
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.880
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.200	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	21731
<I/σ(I)>	15.7	2.6
Completeness [%]	97.3	87.7
Redundancy	3.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	283	4% PEG3350, 0.05M HEPES, pH 8.0, 10mM BTP, 12.5mM NaCl, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 283K, pH 8.00
1		8	283	4% PEG3350, 0.05M HEPES, pH 8.0, 10mM BTP, 12.5mM NaCl, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 283K, pH 8.00
1		8	283	4% PEG3350, 0.05M HEPES, pH 8.0, 10mM BTP, 12.5mM NaCl, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 283K, pH 8.00