2B2A
Crystal Structure of the TEN domain of the Telomerase Reverse Transcriptase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000, 0.953725, 0.97957, 0.97976 |
| Spacegroup name | P 31 |
| Unit cell lengths | 111.100, 111.100, 42.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.710 - 2.220 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.109 | 0.622 |
| Number of reflections | 28712 | |
| <I/σ(I)> | 15.5 | 2.1 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 5.3 | 4.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | PEG 2000 MMe, Magnesium Nitrate hexahydrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
