2B23
Human estrogen receptor alpha ligand-binding domain and a glucocorticoid receptor-interacting protein 1 NR box II peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-08-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.372, 80.905, 58.241 |
| Unit cell angles | 90.00, 109.68, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.2172 |
| Rwork | 0.214 |
| R-free | 0.27211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zky |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.392 |
| Number of reflections | 30529 | |
| <I/σ(I)> | 13.965 | 2.318 |
| Completeness [%] | 94.5 | 94.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.50 |
