2B1X
Crystal structure of naphthalene 1,2-dioxygenase from Rhodococcus sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.744, 144.246, 186.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.800 - 2.000 |
| R-factor | 0.19127 |
| Rwork | 0.191 |
| R-free | 0.22431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ndo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.494 |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.800 | 2.051 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.540 |
| Number of reflections | 147609 | |
| <I/σ(I)> | 7.4 | 1.1 |
| Completeness [%] | 92.9 | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 279 | 0.1 M HEPES, PH 7.8, 68% MPD, 40 MG/ML PROTEIN, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
