2B0S
Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with MN peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.471, 60.471, 275.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.010 - 2.300 |
| R-factor | 0.21926 |
| Rwork | 0.217 |
| R-free | 0.26665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fab |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.458 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 23835 | |
| <I/σ(I)> | 22 | 3.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% PEG 400, 0.2M potassium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
