2B04
Crystal Structure of Porcine Pancreatic Phospholipase A2 in Complex with Glycochenodeoxycholate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-19 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.156, 69.156, 67.043 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.070 - 2.500 |
Rwork | 0.211 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.421 |
Number of reflections | 12928 | |
<I/σ(I)> | 25 | 2.4 |
Completeness [%] | 98.2 | 99.7 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2 M Calcium Chloride, 28% PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |