2AYO

Structure of USP14 bound to ubquitin aldehyde

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.976
Spacegroup name	P 31 2 1
Unit cell lengths	183.931, 183.931, 45.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.000 - 3.500
R-factor	0.29
Rwork	0.290
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 2AYN
RMSD bond length	0.013
RMSD bond angle	1.910
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	3.600
High resolution limit [Å]	3.500	3.500
Number of reflections	11099
Completeness [%]	98.0	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	Tris, calsium chloride, PEG1000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K