2AYB
Crystal structure of HPV6a E2 DNA Binding Domain bound to a 16 base pair DNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.548 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.247, 75.247, 97.426 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.230 - 3.200 |
| R-factor | 0.259 |
| Rwork | 0.189 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Protein of PDB entry 1R8H DNA of PDB entry 1JJ4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.148 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.230 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.138 | 0.387 |
| Number of reflections | 4928 | |
| <I/σ(I)> | 19.8 | 5.2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 22.5 | 22.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | tri-Sodium citrate dihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | tri-Sodium citrate dihydrate | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | tri-Sodium citrate dihydrate | ||
| 4 | 1 | 2 | H2O |
