2AXM
HEPARIN-LINKED BIOLOGICALLY-ACTIVE DIMER OF FIBROBLAST GROWTH FACTOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-08 |
| Detector | FUJI |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.100, 91.100, 193.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.000 - 3.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.100 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.183 | 0.487 |
| Number of reflections | 9682 | |
| <I/σ(I)> | 3.3 | 1.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 10.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | PROTEIN/HEPARIN COMPLEX WAS CRYSTALLIZED FROM 25% PEG 8000, 200 MM MGSO4, 100 MM HEPES, PH 7.0; CRYSTAL WAS SOAKED IN 22% XYLITOL PRIOR TO DATA COLLECTION. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20 (%) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
