2AWG
Structure of the PPIase domain of the Human FK506-binding protein 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-04 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.911, 53.795, 30.640 |
Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
Resolution | 30.890 - 1.600 |
R-factor | 0.16874 |
Rwork | 0.167 |
R-free | 0.20224 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q1c |
RMSD bond length | 0.010 |
RMSD bond angle | 1.355 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.890 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.049 | 0.183 |
Number of reflections | 12477 | |
<I/σ(I)> | 37.2 | 6.75 |
Completeness [%] | 94.0 | 66.4 |
Redundancy | 6.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 1.8M ammonium sulfate, 0.2M sodium acetate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.50 |