2AWD
Crystal structure of LacC from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | .979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.209, 62.236, 91.972 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.19612 |
| Rwork | 0.193 |
| R-free | 0.24617 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.432 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.264 |
| Number of reflections | 48795 | |
| <I/σ(I)> | 9.9 | 2.36 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.4M NaCitrate, .1M Tris pH 8.5, cryo-Xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
