2AVT
Crystal structure of the beta subunit from DNA polymerase of Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.036, 74.666, 82.779 |
| Unit cell angles | 90.00, 118.57, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| Rwork | 0.245 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 30% PEG 2000 MME, 100 mM sodium acetate pH 4.5, 200 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
