2ATX
Crystal Structure of the TC10 GppNHp complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.690, 82.200, 114.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.650 |
| R-factor | 0.21279 |
| Rwork | 0.210 |
| R-free | 0.25994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TC10 GDP (unpublished) |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.755 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 12449 | |
| <I/σ(I)> | 23.34 | 5.95 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MES, PEG 3350, NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
