2AT3
1.00 A Crystal Structure Of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Imidazole at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.751 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.221, 42.743, 52.886 |
| Unit cell angles | 90.00, 94.09, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.000 |
| R-factor | 0.145 |
| Rwork | 0.144 |
| R-free | 0.16700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x8o |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.658 |
| Data scaling software | d*TREK (7.2SSIbeta) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.040 | 0.250 |
| Number of reflections | 81947 | |
| <I/σ(I)> | 20.8 | 2.6 |
| Completeness [%] | 97.4 | 88 |
| Redundancy | 3.23 | 1.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium phosphate, imidazole, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
