2ARR
Human plasminogen activator inhibitor-2.[loop (66-98) deletion mutant] complexed with peptide n-acetyl-teaaagmggvmtgr-oh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.952, 103.802, 41.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.700 - 1.550 |
| R-factor | 0.18745 |
| Rwork | 0.186 |
| R-free | 0.21998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jrr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.676 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 56875 | |
| <I/σ(I)> | 20.7 | 2.6 |
| Completeness [%] | 97.4 | 84.5 |
| Redundancy | 7.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | peg 8K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
