2AQ4
Ternary complex of the catalytic core of REV1 with DNA and dCTP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 177.320, 200.906, 55.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.320 |
| R-factor | 0.19892 |
| Rwork | 0.197 |
| R-free | 0.23781 |
| Structure solution method | MAD |
| RMSD bond length | 0.035 |
| RMSD bond angle | 3.047 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.112 |
| Number of reflections | 42757 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 99.3 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP | ||
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP | ||
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
