2APC

Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP-GlcNAc phosphonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-03
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9363
Spacegroup name	P 21 21 21
Unit cell lengths	40.964, 82.499, 102.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.510 - 1.500
Rwork	0.210
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1foa 1fo9
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.510	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	54569
Completeness [%]	96.9	86.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	1 M Tris pH 7.9, 40% PEG 6000, MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K