2AO7
Adam10 Disintegrin and cysteine- rich domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.034, 0.9789, 1.016 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 146.780, 146.780, 146.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.900 |
Rwork | 0.261 |
R-free | 0.28900 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | autoSHARP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.066 |
Number of reflections | 11118 |
<I/σ(I)> | 21.4 |
Completeness [%] | 99.5 |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 10 mM Hepes, 150 mM NaCl, 0.2M Ammonium Sulfate, 30% PEG 4000, soaked in 1 mM AuCl and 20% glycerol (cryoprotectant) , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |