2AMF
Crystal structure of 1-Pyrroline-5-Carboxylate Reductase from Human Pathogen Streptococcus Pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.822, 109.960, 84.158 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.17549 |
| Rwork | 0.173 |
| R-free | 0.21542 |
| Structure solution method | isomorphous to 2AHR |
| Starting model (for MR) | 2ahr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.329 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.061 | 0.326 |
| Number of reflections | 78923 | |
| Completeness [%] | 98.5 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 3.5 M sodium formate, pH 7.0, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
