2AMA
Crystal structure of human androgen receptor ligand binding domain in complex with dihydrotestosterone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.500, 66.300, 70.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| Rwork | 0.214 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2am9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | XNEMO |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.660 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.471 |
| Number of reflections | 19480 | 2484 |
| <I/σ(I)> | 18.39 | 3.36 |
| Completeness [%] | 89.3 | 80.3 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | MgSO4, Pipes, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
