2AM9
Crystal structure of human androgen receptor ligand binding domain in complex with testosterone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-10 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.820, 65.920, 71.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.230 - 1.640 |
| R-factor | 0.199 |
| Rwork | 0.191 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | hARLBD-DHT (in deposition) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.230 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.062 | 0.504 |
| Number of reflections | 30947 | 2434 |
| <I/σ(I)> | 22.02 | 2.98 |
| Completeness [%] | 96.8 | 77 |
| Redundancy | 10.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | Bicine, Na/K tartrate, MgSO4, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
