2AM5

Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-11
Detector	SBC-3
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	40.619, 82.029, 101.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.940 - 1.600
Rwork	0.177
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fo9 1foa
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.940	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	44852
Completeness [%]	98.0	94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	40% PEK 6000, 1M Tris, MnCl2, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K