2AK3
THE THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN MITOCHONDRIAL MATRIX ADENYLATE KINASE AND ITS SUBSTRATE AMP AT 1.85 ANGSTROMS RESOLUTION
Replaces: 1AK3Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.830, 66.990, 155.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.200 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9999.000 * |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 42879 |
| Completeness [%] | 94.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 12 (mg/ml) | |
| 2 | 1 | drop | MOPS | 75 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | 0.1 (%) | ||
| 5 | 1 | drop | AMP | 3 (mM) | |
| 6 | 1 | drop | PEG8000 | 12 (%(w/v)) | |
| 7 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 8 | 1 | reservoir | MOPS | 75 (mM) |
