2AJR

Crystal structure of Possible 1-phosphofructokinase (EC 2.7.1.56) (tm0828) from Thermotoga Maritima at 2.46 A resolution

Replaces:  1O14

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.885567,0.976224
Spacegroup name	P 41 21 2
Unit cell lengths	115.796, 115.796, 124.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.490 - 2.460
Rwork	0.193
R-free	0.24000
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.274
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SnB
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	2.520
High resolution limit [Å]	2.400	2.460
Rmerge	0.109	0.633
Number of reflections	31322
<I/σ(I)>	5.9	1.2
Completeness [%]	99.5	95
Redundancy	6.3	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	16% PEG-8000, 0.08M sodium cacodylate, 0.16M magnesium acetate tetrahydrate, 20% glyerol anhyrous , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 6.5