2AHM

Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-17
Detector	MARRESEARCH
Wavelength(s)	0.9791, 0.9793, 0.9500
Spacegroup name	P 21 21 2
Unit cell lengths	93.600, 94.000, 150.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.232
Rwork	0.213
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.136
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.099	0.476
Number of reflections	58479
<I/σ(I)>	24	1.7
Completeness [%]	96.8	82
Redundancy	8.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Ammonium Sulfate, Sodium Chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Ammonium Sulfate, Sodium Chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Ammonium Sulfate, Sodium Chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K