2AHM
Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9791, 0.9793, 0.9500 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.600, 94.000, 150.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.232 |
Rwork | 0.213 |
R-free | 0.25100 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.099 | 0.476 |
Number of reflections | 58479 | |
<I/σ(I)> | 24 | 1.7 |
Completeness [%] | 96.8 | 82 |
Redundancy | 8.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Ammonium Sulfate, Sodium Chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Ammonium Sulfate, Sodium Chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Ammonium Sulfate, Sodium Chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |