2AHE
Crystal structure of a soluble form of CLIC4. intercellular chloride ion channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-09-22 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.717, 79.478, 42.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.620 - 1.800 |
R-factor | 0.19618 |
Rwork | 0.194 |
R-free | 0.22941 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1KOM |
RMSD bond length | 0.018 |
RMSD bond angle | 1.536 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.900 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.063 | 0.580 |
Number of reflections | 25137 | |
<I/σ(I)> | 8.3584 | 1.3 |
Completeness [%] | 99.9 | 99.87 |
Redundancy | 4.65 | 4.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |