2AHC
Chorismate lyase with inhibitor Vanilate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.173, 58.560, 70.646 |
| Unit cell angles | 70.24, 67.40, 67.17 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fw9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | X-GEN |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.093 |
| Number of reflections | 33472 | |
| <I/σ(I)> | 18.3 | 6.3 |
| Completeness [%] | 87.0 | 82 |
| Redundancy | 2.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
