2AF1
Structure of the DNA coiled-coil formed by d(CGATATATATAT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-11-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 26.541, 26.541, 220.527 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.000 - 3.100 |
| R-factor | 0.33318 |
| Rwork | 0.332 |
| R-free | 0.34585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gqu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 1099 | |
| Completeness [%] | 99.2 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | cacodylate,MPD, sodium phosphate, potassium chloride, Trimethylamine-N-oxide, spermine , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 10 | 1 | 2 | sodium phosphate | ||
| 11 | 1 | 2 | potassium chloride | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | sodium phosphate | ||
| 4 | 1 | 1 | potassium chloride | ||
| 5 | 1 | 1 | Trimethylamine-N-oxide | ||
| 6 | 1 | 1 | spermine | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | cacodylate | ||
| 9 | 1 | 2 | MPD |
