1GQU
Crystal structure of an alternating A-T oligonucleotide fragment with Hoogsteen base pairing
Summary for 1GQU
| Entry DOI | 10.2210/pdb1gqu/pdb |
| Descriptor | DNA (5'(*AP*TP*AP*UP*AP*T)-3') (2 entities in total) |
| Functional Keywords | dna, dna/rna, new dna structure, hoogsteen base pairing |
| Total number of polymer chains | 4 |
| Total formula weight | 7488.44 |
| Authors | Abrescia, N.G.A.,Thompson, A.,Huynh-Dinh, T.,Subirana, J.A. (deposition date: 2001-12-05, release date: 2002-12-05, Last modification date: 2024-05-08) |
| Primary citation | Abrescia, N.G.A.,Thompson, A.,Huynh-Dinh, T.,Subirana, J.A. Crystal Structure of an Antiparallel DNA Fragment with Hoogsteen Base Pairing Proc.Natl.Acad.Sci.USA, 99:2806-, 2002 Cited by PubMed Abstract: We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA(Br)UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible. PubMed: 11880632DOI: 10.1073/PNAS.052675499 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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