2AE0
Crystal structure of MltA from Escherichia coli reveals a unique lytic transglycosylase fold
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934, 0.9794, 0.9792, 0.9393 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.873, 103.873, 109.777 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.124 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.049 | 0.501 |
Number of reflections | 46854 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 280 | PEG 8000, sodium chloride, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 280K |