2AD6
crystal structure of methanol dehydrogenase from M. W3A1 (form C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-02 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.583, 61.947, 123.804 |
| Unit cell angles | 90.00, 123.30, 90.00 |
Refinement procedure
| Resolution | 33.620 - 1.500 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.051 | 0.132 |
| Number of reflections | 191743 | |
| Completeness [%] | 93.9 | 65.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macroseeding | 8.25 | 293 | Tris-HCl, PEG 8000, methanol, pH 8.25, macroseeding, temperature 293K |
