2ACZ

Complex II (Succinate Dehydrogenase) From E. Coli with Atpenin A5 inhibitor co-crystallized at the ubiquinone binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	170
Detector technology	CCD
Collection date	2003-05-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	H 3 2
Unit cell lengths	138.759, 138.759, 521.873
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.100
Rwork	0.264
R-free	0.30800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nek
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.160
High resolution limit [Å]	3.100	3.100
Number of reflections	34114
<I/σ(I)>	14.7	2
Completeness [%]	95.4	86
Redundancy	4.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	293	Tris-HCl, cacium chloride, Peg 400, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K