2ACX
Crystal Structure of G protein coupled receptor kinase 6 bound to AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.164, 59.270, 221.095 |
| Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OMW (GRK2) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.755 |
| Data reduction software | MOSFLM |
| Data scaling software | ELVES |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 43666 | |
| <I/σ(I)> | 9.3 | 1.2 |
| Completeness [%] | 97.4 | 90.7 |
| Redundancy | 3.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 277 | peg 3350, MALIC ACID, sODIUM CHLORIDE, MAGNESIUM CHLORIDE, amppnp, ethylene glycol , pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
