2AC5

Structure of human Mnk2 Kinase Domain mutant D228G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-23
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 32 2 1
Unit cell lengths	104.646, 104.646, 73.363
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.200
R-factor	0.306
Rwork	0.238
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.300
High resolution limit [Å]	3.200	3.200
Number of reflections	7776
Completeness [%]	98.2	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	290	PEG, pH 7.5, VAPOR DIFFUSION, temperature 290K