2ABW
Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.042, 67.917, 70.327 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.620 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.18920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1R9G CHAIN A |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.962 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.630 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.064 | 0.466 |
| Number of reflections | 52658 | |
| <I/σ(I)> | 19.25 | 2.25 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 30% PEG200, 5% PEG8000 buffered with 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
