2AAQ

Crystal Structure Analysis of the human Glutahione Reductase, complexed with GoPI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-22
Detector	MARRESEARCH
Wavelength(s)	0.902691
Spacegroup name	C 1 2 1
Unit cell lengths	120.310, 62.720, 83.990
Unit cell angles	90.00, 122.47, 90.00

Refinement procedure

Resolution	14.980 - 2.600
R-factor	0.201
Rwork	0.201
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.800
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.760
High resolution limit [Å]	2.600	2.600
Number of reflections	16373
Completeness [%]	99.3	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K