2A8Y
Crystal structure of 5'-deoxy-5'methylthioadenosine phosphorylase complexed with 5'-deoxy-5'methylthioadenosine and sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.597, 96.556, 96.628 |
| Unit cell angles | 91.57, 91.23, 91.52 |
Refinement procedure
| Resolution | 48.270 - 1.450 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cg6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 579262 | |
| <I/σ(I)> | 13.3 | 3.7 |
| Completeness [%] | 94.2 | 90.1 |
| Redundancy | 1.6 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | ammonium sulfate, Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
