2A8N

Biochemical and Structural Studies of A-to-I Editing by tRNA:A34 Deaminases at the Wobble Position of Transfer RNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	200
Detector technology	CCD
Collection date	2003-03-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794, 0.9792, 0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	48.051, 56.337, 90.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.600
Rwork	0.225
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.210
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.600
Rmerge	0.069
Number of reflections	31216
<I/σ(I)>	15.7
Completeness [%]	94.0
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	278	PEG6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K