2A72
Structure of the regulator of G-protein signaling domain of RGS7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-13 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.915, 106.033, 52.490 |
| Unit cell angles | 90.00, 103.71, 90.00 |
Refinement procedure
| Resolution | 53.000 - 2.000 |
| R-factor | 0.18756 |
| Rwork | 0.184 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FQJ_B.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.237 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.434 |
| Number of reflections | 19896 | |
| <I/σ(I)> | 14.9 | 2.2 |
| Completeness [%] | 95.9 | 81.2 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG 3350, (NH4)2SO4, BIS-TRIS, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
