2A72
Structure of the regulator of G-protein signaling domain of RGS7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-13 |
Detector | RIGAKU RAXIS HTC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.915, 106.033, 52.490 |
Unit cell angles | 90.00, 103.71, 90.00 |
Refinement procedure
Resolution | 53.000 - 2.000 |
R-factor | 0.18756 |
Rwork | 0.184 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1FQJ_B.pdb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.237 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.930 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.068 | 0.434 |
Number of reflections | 19896 | |
<I/σ(I)> | 14.9 | 2.2 |
Completeness [%] | 95.9 | 81.2 |
Redundancy | 3.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG 3350, (NH4)2SO4, BIS-TRIS, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |