2A6E

Crystal structure of the T. Thermophilus RNA polymerase holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-14
Detector	ADSC QUANTUM 315
Spacegroup name	P 32
Unit cell lengths	239.500, 239.500, 253.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.231
Rwork	0.231
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iw7
RMSD bond length	0.016
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.066	0.436
Number of reflections	366401
<I/σ(I)>		2.7
Completeness [%]	92.0	77.5
Redundancy	3.05	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	MG FORMATE, PEG400, SPERMIDINE, TRIS HCL, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K