2A69
Crystal structure of the T. Thermophilus RNA polymerase holoenzyme in complex with antibiotic rifapentin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 32 |
Unit cell lengths | 239.500, 239.500, 253.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw7 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.880 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.065 | 0.463 |
Number of reflections | 517106 | |
<I/σ(I)> | 2.35 | |
Completeness [%] | 92.6 | 81.9 |
Redundancy | 3.37 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | MG FORMATE, PEG400, SPERMIDINE, TRIS HCL, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K |