2A5Y

Structure of a CED-4/CED-9 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9645
Spacegroup name	P 41 21 2
Unit cell lengths	128.898, 128.898, 209.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.249
Rwork	0.249
R-free	0.27700
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.490
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	54599
Completeness [%]	99.1	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	PEG3350, HEPES, Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	PEG3350, HEPES, Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K