2A5U
Crystal Structure of human PI3Kgamma complexed with AS605240
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0716 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.446, 67.406, 105.971 |
| Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
| Resolution | 19.580 - 2.700 |
| R-factor | 0.285 |
| Rwork | 0.279 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.410 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.266 |
| Number of reflections | 28933 | |
| <I/σ(I)> | 7 | 2.3 |
| Completeness [%] | 96.5 | 98.5 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 300 | 22% PEG 4K, 100mM Tris-HCl, 200mM (NH4)2SO4, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
