2A5T

Crystal Structure Of The NR1/NR2A ligand-binding cores complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.010
Spacegroup name	P 21 21 21
Unit cell lengths	54.640, 89.989, 126.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.000
R-factor	0.214
Rwork	0.213
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Number of reflections	44066
Completeness [%]	86.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	PEG 8000, HEPES, Calcium acetate, pH 7., EVAPORATION, temperature 277K