2A5S

Crystal Structure Of The NR2A Ligand Binding Core In Complex With Glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	52.106, 52.106, 198.675
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.190 - 1.700
R-factor	0.1961
Rwork	0.196
R-free	0.22300
Structure solution method	Combination of MR and SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.700
Number of reflections	30725
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	PEG 8000, HEPES, calcium acetate, pH 7, EVAPORATION, temperature 277K