2A43
Crystal Structure of a Luteoviral RNA Pseudoknot and Model for a Minimal Ribosomal Frameshifting Motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91980 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.250, 53.250, 55.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.600 - 1.340 |
| Rwork | 0.114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 437d |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.041 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 1.400 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.064 | 0.497 |
| Number of reflections | 19756 | |
| <I/σ(I)> | 24.58 | 4.97 |
| Completeness [%] | 96.0 | 84.9 |
| Redundancy | 14.6 | 7.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | PEG 8000, Magnesium Acetate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
