2A2J
Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2003-08-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.609, 91.108, 92.992 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.480 - 2.500 |
R-factor | 0.21281 |
Rwork | 0.209 |
R-free | 0.26018 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g79 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.007 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.480 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 21094 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Tris/Maleate, PEG 10000, N,N-dimethyldodecylamine N-oxide, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |