2A2C
x-ray structure of human N-acetyl galactosamine kinase complexed with Mg-ADP and N-acetyl galactosamine 1-phosphate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97926, 0.97940, 0.96411 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.800, 123.800, 60.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 59826 | |
| <I/σ(I)> | 46.3 | 3.5 |
| Completeness [%] | 94.3 | 72.7 |
| Redundancy | 8.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG-3400, MES, NaCl, Mg-ATP, N-acetyl galactosamine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
