2A1A
PKR kinase domain-eIF2alpha Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.300, 84.300, 165.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.510 - 2.800 |
| R-factor | 0.21286 |
| Rwork | 0.207 |
| R-free | 0.26837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Q46 for chain A and a PKR homology model (based on PDB entries 2CPK 1bkx 1fmo 1ATP and 1BX6 for chain B. |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.444 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 17397 | |
| <I/σ(I)> | 24.3 | 3.2 |
| Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
