2A0U
Crystal structure of the eukaryotic initiation factor 2B from Leishmania major at 2.1 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979359 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 103.733, 164.569, 172.129 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.300 - 2.100 |
R-factor | 0.21631 |
Rwork | 0.214 |
R-free | 0.26378 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.989 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.300 | 2.213 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 34856 | |
<I/σ(I)> | 8.7 | 1.6 |
Completeness [%] | 85.4 | 63.13 |
Redundancy | 2.9 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.1 M Sodium thiosulfate pentahydrate,0.1 M MOPS 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |